FastQCFastQC Report
Sun 14 Apr 2019
SRR6575814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312393
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9280.2970617139308499No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5720.18310269436254972No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4950.1584542547368219No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4520.14468954169907774No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4100.13124493826686257No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4100.13124493826686257No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4080.1306047190558047No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3970.12708351339498644No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3920.12548296536734177No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3670.11748022522911844No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3450.11043781390748192No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3390.10851715627430833No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3380.10819704666877938No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3380.10819704666877938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.10403562179690326No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3240.10371551219137433No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3200.10243507376925859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10300.038.809541
GGGTACC2600.036.1522457
CTAATAG651.411502E-436.1522453
TCAACGC10750.035.8495754
AACGCAG10900.034.9250566
CAACGCA11000.034.607565
ATCAACG11150.034.5634963
GTAGGAC1107.6686774E-834.1803053
ACATGGG15700.032.928483
GTACATG16050.032.8170661
TGGGTAC2750.032.471296
TACATGG16250.032.1083342
ACAACCC753.270974E-431.3319475
TAATAGT753.270974E-431.3319474
CTCGATA600.004157287531.33194794
TACACCG600.004157287531.3319475
TAGGACT902.5887115E-531.3319454
GGTACCT3100.030.321248
ACGCAGA12600.030.2129487
GTGCAAG1252.3416032E-730.0979391
Produced by FastQC (version 0.11.2)