FastQCFastQC Report
Sun 14 Apr 2019
SRR6575811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842764
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11780.1397781585354856No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11590.13752367210749392No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10800.12814975485426525No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10450.1239967535395437No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10430.12375943917870245No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10120.12008106658566335No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8520.1010959177183648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.053.6835331
TCAACGC19200.038.425524
ATCAACG19600.037.8810843
CAACGCA20100.036.704985
AACGCAG20650.035.7294856
GGTATCA5400.035.7276571
GTACATG36100.031.674771
ACCTAAG4200.031.3706251
TACATGG36700.030.6040552
TATCAAC25050.029.8288522
ACATGGG38150.029.8086413
ACGCAGA25600.028.6389797
CGCAGAG27100.027.0714668
TAAGACA6800.026.9511364
TTAAACG909.525351E-426.1065123
CTAAGAC6450.025.4993863
GCAGAGT29800.024.9343059
TCTATAC950.001305636124.7324853
CCTAAGA5700.023.9094872
CATGGGG22800.023.7019654
Produced by FastQC (version 0.11.2)