FastQCFastQC Report
Sun 14 Apr 2019
SRR6575811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842764
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11080.1314721559060425No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10840.12862438357594771No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10690.12684452586963849No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10440.12387809635912307No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10160.12055569530734582No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9620.11414820756463256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.048.7943081
GGTATCA6750.041.075521
ATCAACG18350.037.8973583
TCAACGC18450.037.6919484
GGTACCG500.001703976437.590085
CAACGCA19500.035.6623885
AACGCAG19950.035.0934946
GGGTACC5250.032.2200667
GTACATG35600.032.2088051
GGTACCT5500.031.6098388
TACATGG37250.031.1605782
ACATGGG36950.030.6468523
TATCAAC24000.029.3707352
TGGGTAC5700.028.8520366
ACGCAGA24650.028.4022397
ATTATCC5000.028.1925563
ACCTAAG4150.027.1767771
ATGGGTA6600.027.0534675
GCATTAG1057.426596E-526.8532451
CTAAGAC5650.026.6124463
Produced by FastQC (version 0.11.2)