FastQCFastQC Report
Sun 14 Apr 2019
SRR6575810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918911
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12130.1320040787410315No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11600.12623638197823292No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC11370.12373341923211278No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10650.11589805759208455No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10160.11056565869817642No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10100.10991271189484074No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9230.10044498324647327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.054.75771
GGTATCA5900.040.7511371
TCAACGC19800.040.5885354
ATCAACG20200.039.7848053
CAACGCA20650.038.9178245
AACGCAG20750.038.5037736
TATCAAC24500.032.8021622
GTACATG37500.031.428981
GTCTTAG4800.031.428981
CCCTATA600.00416174231.3315012
ACGCAGA25550.031.2701877
TACATGG38300.030.7997652
ACATGGG39150.029.8909723
TAGGTAT3450.028.6070215
CGCAGAG28050.028.4831818
TTAGGTA3900.026.5112724
ATGGGAG9500.026.219525
GCAGAGT30850.026.202689
AGGTATA3600.026.1095856
TACGCTA4950.025.6348659
Produced by FastQC (version 0.11.2)