FastQCFastQC Report
Sun 14 Apr 2019
SRR6575809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882525
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14590.165321095719668No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14140.1602220900257783No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12570.14243222571598538No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11830.1340471941304779No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11210.12702189739667433No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10620.12033653437579672No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10390.11773037591003088No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9660.10945865556216537No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9550.10821223194810345No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT9340.10583269595762161No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9250.10481289481884366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.005235512756.4003371
GTATCAA14700.047.9594731
GGTATCA6750.036.2076261
TCAACGC20450.034.2350044
GTACATG38850.033.511141
ATCAACG20950.033.417943
CAACGCA21100.033.4030575
AACGCAG21650.032.771516
TACATGG40150.032.1919752
ACATGGG40750.031.1324673
AGGTATA2300.030.6436756
TAGGTAT2350.029.9916845
AGACCGT1602.1391315E-929.3668566
TATCAAC25400.028.4962332
ACGCAGA25900.027.3939467
TTAGGTA2600.027.1078644
ATTATCC4600.026.5578523
GACCGTC1258.038443E-626.3127027
GTCTTAG3300.025.6365171
CATGGGG21900.025.5317334
Produced by FastQC (version 0.11.2)