FastQCFastQC Report
Sun 14 Apr 2019
SRR6575808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960928
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14880.15485031136567984No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13200.13736721169536115No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13140.13674281527856408No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11870.12352642445635886No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11750.12227763162276466No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11060.11509707282959805No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10620.11051816577308603No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10060.10469046588297978No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9850.10250507842418995No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9760.1015684837989943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18850.045.7357981
GTATACG500.00170144437.6019974
GGTATCA8900.036.523771
TCAACGC25050.033.9618844
CAACGCA25450.033.4281045
ATCAACG25600.033.4175763
AACGCAG26500.032.280966
GTACATG43100.031.370481
TACATGG43300.031.1557032
ACATGGG44700.029.9695953
TAGGTAT2002.7284841E-1128.2014985
TATCAAC30550.028.0029452
ACGCAGA30850.027.4216187
CGCAGAG33050.025.5962758
CATGGGG23700.025.186994
CTAAGAC4300.025.1421783
ACCTAAG3000.025.125531
TTAGGTA3050.024.6570474
AGGTATA2501.6370905E-1124.4412986
GTATAGT3100.024.2568288
Produced by FastQC (version 0.11.2)