FastQCFastQC Report
Sun 14 Apr 2019
SRR6575808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960928
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15230.15849262379699622No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14850.1545381131572813No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13670.14225831696027175No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11860.12342235838689267No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11690.12165323520596756No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11490.11957191381664391No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10970.11416047820440242No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10940.11384827999600386No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10760.11197509074561257No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT10040.10448233374404743No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9720.10115221952112959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.055.9570271
GGTATCA6350.041.452261
ATCAACG24050.038.8889963
TCAACGC24100.038.6132974
CAACGCA25300.037.153375
AACGCAG26050.036.083696
TATCAAC28950.032.4724732
GTACATG44450.031.4064331
TACATGG45100.031.1622332
ACGCAGA31000.030.3219437
ACATGGG46250.030.2826063
GTCTTAG4650.028.3034251
CGCAGAG33450.028.1010538
GTATATG1205.838103E-627.4189411
TTAGGTA2750.027.344884
CCCCCCC4400.025.63582494
CATGGGG24250.025.3891564
GCAGAGT37600.024.9994759
GCTATAT1701.2424243E-724.8844181
AGAGTAC32850.023.89295210-11
Produced by FastQC (version 0.11.2)