FastQCFastQC Report
Sun 14 Apr 2019
SRR6575804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101688
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16760.15213018567870396No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12900.11709304267632942No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12070.10955914923281365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19750.057.0503581
GGTATCA7150.044.8363721
TCAACGC25250.043.3690224
ATCAACG25700.042.9753883
CAACGCA27250.040.5309145
AACGCAG27700.039.872476
TATCAAC30250.037.288162
GTCTTAG5350.033.4855461
ACGCAGA34050.032.4351657
TAGGTAT4750.031.6622265
ACGTAAC600.00409901831.4294151
CGCAGAG36300.030.5541888
GTACATG49900.030.2327041
TACATGG51650.029.027222
CCGTATA650.00615494428.92222494
CTTAGGT4900.028.7746623
ACATGGG51550.028.6276723
GCAGAGT39900.028.2685629
CGTAACA700.00883713426.856352
AGGTATA5100.026.7247036
Produced by FastQC (version 0.11.2)