FastQCFastQC Report
Sun 14 Apr 2019
SRR6575804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101688
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15830.14368859422994532No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13210.11990690649258229No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11690.10610989681289076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19200.056.0712051
TCAACGC25650.040.860614
ATCAACG25400.040.7076763
GGTATCA7200.039.176391
CAACGCA27100.038.847775
AACGCAG28250.037.4327246
AGGTATA4000.036.4241186
TATCAAC29000.035.988242
TTAGGTA4150.035.1075864
TAGGTAT4250.034.2815255
GTCTTAG5800.032.421841
GTACATG48300.031.8277761
GGTATAG4900.031.6522987
TACATGG49900.031.3725152
ACATGGG50050.030.8004573
CTTAGGT4800.030.3534343
ACGCAGA34400.030.3306647
CGCAGAG37300.028.0985188
GCAGAGT40350.026.3240249
CATGGGG29200.024.9480294
Produced by FastQC (version 0.11.2)