FastQCFastQC Report
Sun 14 Apr 2019
SRR6575801_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575801_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395884
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA21790.1561017964243447No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17510.1254402228265386No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16740.11992400514655946No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16580.11877777809617418No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15620.11190041579386253No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15400.11032435359958276No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15090.1081035386894613No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14640.10487977511025272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.054.5437771
GGTATCA7000.042.405351
ATCAACG24400.041.9923323
TACGATC450.001013967941.77835
TCAACGC24650.041.757124
CAACGCA25400.040.3390925
AACGCAG26700.038.0229536
CTTAGGT6000.036.0337873
ATAAGCG550.00271703934.1822475
GTCTTAG8000.031.8040121
GTACATG53100.031.76631
TATCAAC33050.031.4285342
TAAGACA13050.031.3337254
TTAGGTA6800.031.1033334
CTAAGAC12300.030.569493
TACATGG54850.030.5053982
ACCTAAG9600.030.4297621
TAGGTAT6800.030.4121485
ACATGGG55200.030.3119773
ACGCAGA34400.029.6465057
Produced by FastQC (version 0.11.2)