FastQCFastQC Report
Sun 14 Apr 2019
SRR6575798_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575798_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343638
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29850.22215805149899004No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23840.1774287419677026No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19270.1434166047700348No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15330.11409323046832555No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14500.1079159714149198No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14390.10709729852832386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21650.053.738341
TCAACGC26900.042.974284
ATCAACG27100.042.4837273
CAACGCA27900.041.6024175
AACGCAG29700.039.713956
GGTATCA8700.036.2744451
ACGCAGA34450.033.9653367
TATCAAC34500.033.64622
CGCAGAG37850.031.1834858
GTACATG53700.030.3491841
TACATGG54600.029.9533042
ACATGGG55550.028.8466993
GCAGAGT42100.028.0355119
ACCTAAG11350.024.9000721
TAAGACA14850.024.366334
AGAGTAC36500.023.38301810-11
GTCTTAG6900.023.2099931
CATGGGG32450.023.170274
GTACTTG9400.023.0502261
CTTAGGT6150.022.9230253
Produced by FastQC (version 0.11.2)