FastQCFastQC Report
Sun 14 Apr 2019
SRR6575792_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575792_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658889
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12390.1880438131460686No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA8520.129308578531437No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7320.11109610268193884No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7180.10897131383283071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.10851600193659326No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6730.1021416353892689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.056.400061
ATCAACG18650.039.818563
TCAACGC18900.039.7862054
CAACGCA19100.038.8774765
AACGCAG19900.037.3145646
ATCACGC550.00271588134.1825643
TATCAAC22200.033.6628952
GGTATCA5050.033.589811
ACGCAGA22600.032.6486787
GTACATG35300.032.569531
ACATGGG34900.031.9176033
TACATGG36100.031.8982052
CGCAGAG23400.031.5324848
CATGGGG17600.028.5723174
ATGGGAG7350.028.1345355
GCAGAGT26400.027.9492479
CATGGGA13100.027.26574
GTATAAT3350.026.7242281
ATGGGGA9500.024.7355045
AGAGTAC25650.024.09421210-11
Produced by FastQC (version 0.11.2)