FastQCFastQC Report
Sun 14 Apr 2019
SRR6575791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619180
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24510.15137291715559725No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22050.13618004174952753No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18840.1163551921342902No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17700.10931459133635543No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17060.10536197334453243No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16480.10177991328944282No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16230.10023592188638693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24650.051.3734441
GGTATCA10000.040.4857141
TCAACGC31050.039.6519434
ATCAACG31250.039.398173
CAACGCA32800.037.6796385
AACGCAG34200.036.5494046
GTACATG59800.030.7020071
TATCAAC41850.029.5332972
ACGCAGA42050.029.5027667
TACATGG63200.028.9257182
ACATGGG65800.027.4953753
CGCAGAG46650.026.5100568
GTCTTAG8350.026.49811
TAACTCG909.528715E-426.1067184
AGGTATA6850.025.3825916
ACCTAAG10400.025.3488371
TAGGTAT6600.024.9200485
CTAAGAC12600.024.6149083
GCAGAGT51750.024.1700659
AGAGTAC42700.024.11697210-11
Produced by FastQC (version 0.11.2)