FastQCFastQC Report
Sun 14 Apr 2019
SRR6575788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833887
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA36820.44154663641476605No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21840.2619059896604696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.20290518979190225No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15270.18311833617744372No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15020.1801203280540409No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13680.16405100451260182No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11650.139707178550571No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11530.13826813465133767No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG11110.1332314810040209No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG9810.11764183876232631No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC9650.1157231135633485No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8910.1068490095180762No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8750.10493028431909839No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG8400.10073307294633446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC503.009799E-546.997123
TAGTATG1400.040.283257
GTGCAAG3100.039.5592651
TCTAACG757.4442687E-637.59772
GTATCAA54350.034.9737361
ATGGGAG10050.033.201955
ACCTAAG13250.033.1057661
GTAATCA3550.031.7727019
GGTAATC3450.031.3314138
CTAAGAC14250.031.3314133
TAGTACT1950.031.3314134
TAAGACA14550.031.008414
CATGGGG29250.030.5280464
CTAACGC953.7526224E-529.6823923
AAGGGTA3800.029.6823925
TAGGTAT3650.029.6146245
GTACTTG5600.029.4792631
TCGGTAG804.7814834E-429.3732039
CAACGCA66050.028.8173125
ATCAACG65750.028.8058413
Produced by FastQC (version 0.11.2)