FastQCFastQC Report
Sun 14 Apr 2019
SRR6575781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107571
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2510.23333426295191081No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.22775655148692492No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2220.2063753242044789No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1690.1571055395971033No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1600.14873897239962441No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1410.13107621942716904No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1320.12270965222969014No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1220.11341346645471362No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1210.11248384787721596No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1190.11062461072222068No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1160.10783575498972771No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1110.10318766210223945No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1100.10225804352474178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA156.892654E-493.973963
TGCCCGC504.5333036E-756.384381
CAAACGC352.9295337E-453.699412
TAGTACT550.00270980134.172354
GTATCAA7650.033.781491
ATGGGTA753.261285E-431.3246565
TTATTCC753.261285E-431.3246564
AGTACTG600.004150009731.3246545
CATGGGG3500.030.877164
CATGGGT1859.094947E-1230.4780434
GTACATG11150.029.919961
TACATGG11200.029.3668652
CCTATAA804.7601713E-429.3668631
AAACGCA650.00613803728.9150643
ACATGGG11400.028.439493
ATGGGGC1005.289405E-528.192195
AACGCAG9000.028.192196
CGGCGTT350.00883227526.84970540-41
GTTCTAC700.008812997526.8497051
GGCAATC350.00883227526.84970582-83
Produced by FastQC (version 0.11.2)