FastQCFastQC Report
Sun 14 Apr 2019
SRR6575780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926923
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12180.13140250053132785No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11520.1242821679902214No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11190.1207220017196682No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10910.11770125458101698No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10440.11263071474113814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.056.5651861
TCAACGC17500.037.5879174
ATCAACG17900.037.010453
CAACGCA18750.035.0820585
AACGCAG19250.034.1708346
GTACATG37650.033.7125321
TACATGG39200.031.8980942
ACATGGG38850.031.807023
TATCAAC22050.030.2725432
ACGCAGA24050.027.3508767
CATGGGG25400.026.4521264
ACCTAAG4800.026.443271
GTCTTAG5200.026.2172581
GGTATCA4700.025.0054551
TAGGTAT3200.024.9607285
CTAAGAC6600.024.9162333
CGCAGAG26550.024.7754618
CTATTAA1901.3291356E-824.7422391
TAAGACA6850.024.6927934
AGAGTAC24350.023.54068810-11
Produced by FastQC (version 0.11.2)