FastQCFastQC Report
Sun 14 Apr 2019
SRR6575779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041348
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12640.12138113291618172No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC10600.10179113994553213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14850.055.1804471
TCAACGC19350.041.0499154
ATCAACG19650.040.4231953
CAACGCA20750.038.2802775
AACGCAG21200.037.0243236
TATCAAC25200.031.1474482
ACGCAGA26500.029.6180367
TAAGACA7450.029.0207124
GTACATG38150.028.6388681
GGTATCA5200.028.0750641
ACATGGG38300.027.9796713
CGCAGAG28400.027.9675248
TACATGG38200.027.8068372
TAGGTAT3400.027.647615
ACCTAAG4900.026.910661
CTAAGAC7250.026.579843
GTCTTAG5500.026.5436971
TTAGGTA4150.026.0487144
AGGTATA3650.025.7539376
GTACTAT1659.138057E-825.6874471
Produced by FastQC (version 0.11.2)