FastQCFastQC Report
Sun 14 Apr 2019
SRR6575778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988931
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA23200.23459675144170827No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18540.18747516257453756No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13710.13863454578731985No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13430.135803205683713No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13200.13347746202717886No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12690.12832037826703785No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10170.10283831733457643No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10090.10202936301926019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.053.3602681
TCAACGC19200.038.672224
ATCAACG19500.037.836263
CAACGCA20200.036.757755
AACGCAG21050.035.2734686
GTACATG42800.031.8885461
TACATGG43150.031.4793782
CTATGCG902.585047E-531.3484139
TATCAAC24400.030.627842
ACATGGG44950.030.0051213
TAGGTAT4400.028.8372785
ACGCAGA26150.028.394137
CTTAGGT4500.027.1521383
CGCAGAG27850.026.6771058
ACCTAAG8350.026.4906021
CTAAGAC9650.025.810223
CATGGGG26600.025.7937454
ATTATCC9000.025.5856693
AGGTATA4600.025.5402626
TTAGGTA5000.025.3768044
Produced by FastQC (version 0.11.2)