FastQCFastQC Report
Sun 14 Apr 2019
SRR6575775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240463
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14930.1203582855756278No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14130.1139090807222787No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13080.10544449935225798No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12760.10286481741091835No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12500.10076882583357988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16750.052.6073841
TCAACGC22050.039.221554
ATCAACG22550.038.1434633
CAACGCA22700.037.47735
AACGCAG24600.034.7737776
GTACATG46050.031.414391
TACATGG46650.030.5285762
ACATGGG49150.028.880123
TATCAAC29950.028.8759672
ACGCAGA31150.027.1578277
ACCTAAG5900.026.3561381
CATGGGG32550.025.2698484
CGCAGAG34500.025.0656668
CTTAGGT3650.024.466753
TAAGACA9200.023.500964
TAGGTAT3800.023.5009575
GCAGAGT37400.022.9964089
GGTATCA6500.022.4733721
CTAAGAC9250.022.3576683
GTATTAC1903.5507583E-722.3207511
Produced by FastQC (version 0.11.2)