FastQCFastQC Report
Sun 14 Apr 2019
SRR6575767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802708
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28000.34881924684941473No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19310.24056070202364993No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC16410.20443299431424627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11690.14563203555963064No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10180.1268207118902515No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG9900.12333251942175734No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9890.12320794111931113No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9810.12221131469974138No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9600.11959517034837078No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9150.11398914673829089No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8820.10987806275756563No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8730.10875685803554966No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8590.10701276180130259No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8510.10601613538173282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.040.1972351
ACCGTCC500.001704121737.589218
AAGGGTA2150.037.1521265
CTTAGGT5650.034.928033
TTAGGTA5600.034.400844
AGGTATA5650.034.096416
TAGGTAT5700.033.7973185
GTATAGA851.7553262E-533.173151
TCAACGC30100.031.6885814
CAACGCA30400.031.3758625
ATCAACG30750.031.0187383
GGTATAG6250.030.8231537
AACGCAG31100.030.8207366
ACCTAAG9500.029.681241
GTCTTAG8450.028.9201811
CTAAGAC11750.028.391853
ATTTAGG856.8182714E-427.6442931
TATCAAC34650.027.2613412
GGTAATC2600.027.1076038
CATGGGG26000.026.746174
Produced by FastQC (version 0.11.2)