FastQCFastQC Report
Sun 14 Apr 2019
SRR6575763_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575763_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680970
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27910.4098565281877322No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23970.3519978853693995No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10420.15301701983934682No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10070.147877292685434No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9880.14508715508759565No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9720.1427375655315212No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9290.13642304359957122No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA9280.13627619425231655No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9140.13422030339075144No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9010.13231126187644096No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8700.12775893211154676No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8570.12584989059723628No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8300.12188495822136071No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8190.12026961540155955No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8130.11938851931803164No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8110.11909482062352232No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7990.11733262845646651No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7320.10749372219040487No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT7280.10690632480138626No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7080.10396933785629323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAG2000.032.907741
CATGGGG19550.029.802434
CTAACTG2450.028.7674854
CCTAAGA2500.028.2066352
ATGGGGG11800.027.8735835
TAATAAT3450.025.8768393
GTCTTAA1101.01611346E-425.6423931
ATGGGTA2651.8189894E-1224.8232635
ACGTTAA950.001306160724.7299448
CTATTGA4800.024.472349
AGGGCTA5250.024.1646865
GCTTTAG1000.00175554123.505531
TAACCAC2002.3015673E-823.4934486
TGGGGGG6200.023.4934466
TAAGACA4050.023.2034034
TATTCTC2853.6379788E-1223.0812825
ATACCTT1452.5367735E-522.6833275
CAGACTA3350.022.4415029
TAGGAAC1050.002338310722.3747124
GGGTACC2756.002665E-1122.2119877
Produced by FastQC (version 0.11.2)