FastQCFastQC Report
Sun 14 Apr 2019
SRR6575761_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575761_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592961
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24100.4064348245500126No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20730.3496014071751768No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9020.15211793018427858No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8780.14807044645431994No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8050.1357593501090291No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7700.12985676966950607No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7150.12058128612168423No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7100.1197380603446095No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7040.11872618941211985No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7030.11855754425670491No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6930.11687109270255547No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6840.115353286303821No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6800.11467870568216121No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6760.11400412506050145No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6290.10607780275599912No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6210.10472864151267959No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6180.10422270604643477No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6000.10118709324896577No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT5980.10084980293813589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC1950.036.143537
TAATAAT2750.034.172073
TGGGTAC2200.032.0363166
CGTTAAC1551.5133992E-930.3267171
CTTAAGG856.8067375E-427.6508312
GTCTTAC856.8067375E-427.6508311
TTATATC856.823541E-427.6391724
TATTCTC1704.0799932E-927.6391725
GTATATA700.00882675926.8608061
ATATCAC700.00884493226.8494823
AAGGGTA1057.430032E-526.8494825
CATGGGT4500.026.1036624
ATGGGTA2750.025.6290515
GTCCTAC2101.4242687E-924.6224061
CTAACTG2101.4315447E-924.6120244
TTAGGTA1351.4591156E-524.3634194
GGTACCT3100.024.2511448
CATGGGG17650.024.2253844
TCTAACG2151.87174E-924.0497912
CTAACGC2151.880835E-924.0396523
Produced by FastQC (version 0.11.2)