FastQCFastQC Report
Sun 14 Apr 2019
SRR6575758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014413
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12850.12667424411950556No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11530.1136617925834941No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11260.11100015476930994No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10240.10094507858239199No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10150.10005786597766393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.054.4857331
ATCAACG16600.041.3274653
TCAACGC16800.040.835474
CAACGCA17650.039.401345
GGTATCA5050.037.2335051
AACGCAG18750.037.0897946
GTACATG37400.031.9247441
TACATGG38200.031.0115812
ACCTAAG3500.030.890511
TATCAAC23650.030.412142
ACATGGG40200.029.6894173
CTAAGAC5800.028.3552883
ACGCAGA24150.028.2127137
GTCTTAG5300.027.4948371
TGTCGCG700.00884797626.849494
CGTTTAG459.510205E-426.11580890-91
TAAGACA6300.026.104874
CGCAGAG26300.025.9063518
GTACATA1951.753142E-824.1063061
CATGGGG27250.023.7961434
Produced by FastQC (version 0.11.2)