FastQCFastQC Report
Sun 14 Apr 2019
SRR6575755_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575755_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189160
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14190.11932792895825625No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14080.11840290625315349No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13660.11487100137912477No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13560.11403007164721315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.052.6320721
TCAACGC23950.035.3221054
ATCAACG24600.034.579853
CAACGCA24800.034.111475
AACGCAG25850.032.5440866
ATAGACG1201.6529884E-731.3320168
GTACATG49900.031.1620961
TACATGG49750.030.9856322
ACATGGG50150.030.551063
TATCAAC31200.028.319322
TAGACGA1406.370974E-726.8560149
ACGCAGA31950.026.1835657
TAAGACA8100.024.949574
GTTAAGC950.001304931924.7358023
CTAAGAC7250.024.6334483
ACCTAAG5900.023.959761
CGCAGAG34800.023.9041678
CATGGGG30700.023.5755564
TACTAGA1201.8386214E-423.4990122
ATTATCC5800.022.68873
Produced by FastQC (version 0.11.2)