FastQCFastQC Report
Sun 14 Apr 2019
SRR6575754_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575754_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769398
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12590.16363442587581462No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10860.14114931413910617No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10360.134650726931965No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9990.12984177239868053No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9790.12724233751582406No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9710.12620256356268147No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8490.11034601077725703No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG8280.10761660415025773No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7980.10371745182597303No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7770.10098804519897374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.055.1644741
TCAACGC17000.040.0815284
ATCAACG17250.039.5006373
CAACGCA18200.037.438795
AACGCAG18600.036.3810126
ACGCAGA21700.031.1837227
GGTATCA4200.030.276131
TATCAAC23250.029.9132752
GTACATG29500.029.375261
TACATGG30000.028.9784832
TAGGTAT2300.028.6039095
ACATGGG30050.028.4611263
GTCTAGA1005.247628E-528.257721
CTTAGGT2500.028.195283
CGCAGAG24300.027.6735928
GTCTTAG3450.027.3021471
AGGTATA2450.026.8526526
GCAGAGT25400.026.4751289
TATATAC1258.029834E-626.3155943
AGAGTAC22350.025.77474410-11
Produced by FastQC (version 0.11.2)