FastQCFastQC Report
Sun 14 Apr 2019
SRR6575751_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575751_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313305
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17200.13096729244158822No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14200.1081241600389856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18550.054.1195871
ATCAACG26150.037.7477153
TATTACG500.001701245737.6039122
TCAACGC26700.037.144774
CAACGCA28150.035.5654035
AACGCAG29350.034.2714276
CCCTATA1256.2627805E-933.8435252
TATCAAC31550.031.1379492
TAGGTAT4650.030.3245835
TAAGACA10700.030.3104134
ACCTAAG6650.029.7677781
GTACATG50700.029.3763561
TACATGG51100.028.6076762
CTAAGAC10150.028.2492473
TTAGGTA4850.028.1049484
ACATGGG51900.027.7138673
ACGCAGA36500.027.5548347
CGCAGAG37700.026.6787768
AGGTATA4950.026.5876146
GGTATCA7350.026.2914941
Produced by FastQC (version 0.11.2)