FastQCFastQC Report
Sun 14 Apr 2019
SRR6575750_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575750_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212561
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15550.1282409709697079No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14590.12032384350148158No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14540.11991149311251145No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13470.11108719478855084No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12410.10234536654238427No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC12370.10201548623120817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.050.8160631
TCAACGC22950.038.4963574
ATCAACG23200.038.0815283
CAACGCA24850.035.5529755
AACGCAG25450.034.530146
GTACATG47050.031.9280191
TACATGG48350.030.8111062
ACATGGG48700.029.914193
GTCTTAG6550.029.4770131
TATCAAC30950.029.304962
TTAGGTA4200.029.0916654
ACGCAGA30950.028.3927567
TAGGTAT4000.028.1965375
TCTTAGG6250.027.0686762
ACCTAAG5000.026.3711431
CTTAGGT4350.025.927853
CGCAGAG34100.025.9259478
AGGTATA4200.025.7349346
CATATAC2451.2732926E-1124.9357133
ATTATCC7950.024.8271393
Produced by FastQC (version 0.11.2)