FastQCFastQC Report
Sun 14 Apr 2019
SRR6575750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212561
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17350.14308558497263232No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15940.13145730400367486No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15700.1294780221366183No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15230.12560192848029914No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13550.11174695541090304No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12640.10424217833164683No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12620.10407723817605877No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12320.10160313584223804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.054.442731
TCAACGC25150.040.7312164
ATCAACG25550.040.4613723
CAACGCA27150.037.7307625
AACGCAG29350.035.2227676
GTACATG51150.032.717071
TATCAAC32900.031.577982
TACATGG53500.031.1055222
ACATGGG55100.029.8486923
GTCTTAG6050.029.5255721
ACGCAGA35400.029.0691177
CTTAGGT3250.028.917153
CGCAGAG37950.027.1158568
CATGGGG32250.026.9557154
TAGGTAT3550.025.1497755
AGGTATA3850.024.4105836
GCAGAGT42200.024.2726449
TTAGGTA3850.023.1900544
TCTTAGG6900.023.1642272
AGAGTAC36200.022.9740610-11
Produced by FastQC (version 0.11.2)