FastQCFastQC Report
Sun 14 Apr 2019
SRR6575744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865646
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA32800.3789077752337561No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC22200.25645587226187144No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18020.20816823505220378No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12810.1479819695348907No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12180.14070416775448624No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11940.13793167183814167No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9980.11528962185466114No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9920.114596497875575No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9400.1085894233901618No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9110.10523932415791212No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8970.1036220348733778No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8890.1026978695679296No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8730.10084953895703325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33400.042.3645941
GGTAATC3650.034.7659038
ATCAACG40950.034.54583
TCAACGC41450.034.3558544
CAACGCA41850.034.027485
AACGCAG43150.033.111246
TATCAAC44700.031.8653052
GTGCAAG4450.031.6916071
GTCTTAG7050.031.3394781
TGATCGC1052.0651496E-631.3322359
AAGGGTA4250.030.9636215
TCTTAGG7950.030.7481672
AGGGTAA4300.030.603586
AGGTATA5550.030.4854186
GTAATCA4500.030.2878279
CTTAGGT5650.029.9458543
TTAGGTA5900.029.4735454
GTACATG65500.029.3538461
ACGCAGA48700.029.3377847
TAGGTAT5850.028.9220665
Produced by FastQC (version 0.11.2)