FastQCFastQC Report
Sun 14 Apr 2019
SRR6575740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585062
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29670.5071257405198082No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25610.43773138573347786No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11590.19809866304767698No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11130.19023624846597453No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10770.18408305444551176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10070.1721185105168341No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9880.16887099145047874No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9220.157590135746297No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9190.15707736957792506No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG9130.1560518372411813No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8410.1437454492002557No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8330.14237807275126396No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8280.14152346247064412No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8200.14015608602165242No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8050.1375922551797929No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7610.1300716847103384No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC7600.1299007626542144No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7530.12870430826134666No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6860.11725253050104091No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT6660.11383408937856158No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6540.111783024705074No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6340.10836458358259467No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT6300.10768089535809881No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6250.10682628507747897No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC6050.10340784395499963No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5950.10169862339375998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA352.9330747E-453.72632
ACCTAAG2550.035.0274431
AAGGGTA1353.45608E-1034.8136755
AGGGTAA1256.2573235E-933.8388946
GGGTACC851.7537082E-533.175397
GACGTTA1201.6504055E-731.3323067
GGTAATC1354.6294736E-727.8509398
GTTAATA3600.027.4227981
CATGGGG18700.027.1434974
ATTACGC700.00883394926.8562648
ATGGGGG12250.026.8562625
CGTTAAC1659.2812115E-825.6420971
CCTAAGA3500.025.5199952
TATTCTC1851.0018994E-825.4045735
CAAGGGT1851.0018994E-825.4045734
ACGTTAA1501.1612301E-625.0658458
GTGCAAG1901.3269528E-824.7423781
GTATAAG3500.024.1768361
ATAAGGT3700.024.1343443
TAGGACC2151.875378E-924.0457234
Produced by FastQC (version 0.11.2)