FastQCFastQC Report
Sun 14 Apr 2019
SRR6575739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738454
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12170.16480376570510824No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9080.12295958854579973No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8940.12106373585897023No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8640.11700119438719271No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8080.10941778363987464No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8030.1087406933945784No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7760.10508440606997864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12550.053.247491
ATCAACG15950.040.0695763
TCAACGC16200.039.7413024
ACCTAAG5050.038.207351
CAACGCA17250.037.5946925
AACGCAG17700.037.1698956
CTAAGAC5900.036.6388933
TAAGACA6600.034.176994
GGTATCA4700.032.0410421
TATCAAC20650.031.409022
CCTAAGA6700.030.8654942
ACGCAGA21400.030.5237277
AGGTATA2550.029.4860346
TTAGGTA3100.028.8023834
ACCAGAT6950.028.42003694
CGCAGAG23850.027.6020228
GTCTTAG3700.026.7098881
TAGGTAT3000.026.6295725
GCAGAGT25400.025.9176489
GTACATG29800.025.7410261
Produced by FastQC (version 0.11.2)