FastQCFastQC Report
Sun 14 Apr 2019
SRR6575739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738454
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11020.1492306900632944No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10290.1393451724819691No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10030.13582430320642855No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9760.1321680158818288No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9490.12851172855722903No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8680.1175428665834297No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8010.1084698572964599No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7810.10576149631527489No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7760.10508440606997864No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7740.10481356997186014No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7690.10413647972656388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.050.4575461
TCAACGC16700.038.264644
ATCAACG17450.036.8892943
GGTATCA5250.036.7117961
CAACGCA17550.036.4113665
AACGCAG18200.034.8527956
TATCAAC22150.029.287782
ACGCAGA22100.028.7023037
GTACATG32050.028.3080771
TACATGG32300.028.0889742
CGCAGAG22750.028.088778
ACCTAAG4050.027.8571911
TACACCG1057.432312E-526.849565
ACATGGG33650.026.670033
ATAGACG909.530138E-426.1037398
CATGGGG18600.025.7669164
TAGACGA950.001306291924.7298569
GCAGAGT26050.024.53059
TAAGACA5400.024.363494
ATTATCC4750.023.7406643
Produced by FastQC (version 0.11.2)