FastQCFastQC Report
Sun 14 Apr 2019
SRR6575738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808418
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11600.14349012515802467No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10710.13248096900365902No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10330.12778043042089612No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9930.12283249507061941No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9490.11738976618531503No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC9480.1172660678015581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14700.049.900241
ATCAACG17800.040.661293
TCAACGC18000.040.20954
CAACGCA18800.038.9984325
AACGCAG19100.038.878026
TATAGGG550.002710482634.197372
TATCAAC21800.034.0797162
CTTAGGT3150.031.3320773
ACGCAGA23750.031.0682267
GTACATG35850.030.5606171
TACATGG37150.029.9974882
TAGGTAT3500.029.5416725
GGTATCA5450.029.3344381
ACATGGG37650.028.9603253
CGCAGAG25500.028.9360928
TTAGGTA3600.027.4155674
GCAGAGT28100.026.4259859
CATGGGG21600.025.6748964
GGTAATC1851.0031727E-825.4043858
GGTATAG3800.024.735857
Produced by FastQC (version 0.11.2)