FastQCFastQC Report
Sun 14 Apr 2019
SRR6575737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328121
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9060.2761176517199448No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6250.19047851249996192No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5990.18255460637996349No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5110.15573523181996884No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4880.14872562255997024No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4630.14110648205997178No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4620.14080171643997183No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4150.12647773229997472No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3970.12099195113997581No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3970.12099195113997581No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3880.11824906055997635No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3840.11702999807997659No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3810.11611570121997677No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3770.11489663873997702No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3660.11154421691997769No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3640.1109346856799778No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3630.11062992005997788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.10941085757997812No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3430.10453460765997909No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3380.10301077955997939No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3340.10179171707997964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTT250.00523819156.38516
GTATCAA8550.045.0709841
ATAGCAC651.4134853E-436.1442953
ATCAACG10650.035.2958373
AACGCAG10650.035.2958376
TCAACGC10750.034.96754
GTACATG18450.034.6411131
TACATGG18850.033.9060172
CAACGCA11100.033.864925
ACATGGG19500.032.7708243
ATGGGAG3650.032.1832735
TCTGTAC1252.3599387E-730.072053
ACGCAGA12500.029.6961527
GTATGGC650.006151207728.9198441
GTATGAA650.006151207728.9198441
CGCGGTA1802.382876E-1028.71463294
CATGGGG10000.028.6624244
CGCAGAG13200.028.1213558
TTAGGTA1354.6283822E-727.8444944
TATCAAC13900.027.7235162
Produced by FastQC (version 0.11.2)