FastQCFastQC Report
Sun 14 Apr 2019
SRR6575736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031802
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26720.2589644137150345No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21000.20352742095867232No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17810.17261063653685493No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12740.1234733020482612No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11200.10854795784462523No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10750.10418665596693939No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10470.10147295702082376No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10340.10021302536727009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19150.046.683361
TCAACGC24300.036.743214
GGTATCA8000.036.4652331
ATCAACG24600.036.2951243
GTACTAA651.4042402E-436.193781
CAACGCA25600.034.877355
AACGCAG26150.034.1437876
TATCAAC29100.031.0084552
GTACATG44950.030.25141
GTCTTAG7600.029.7171
TACATGG45600.029.2701782
TAGGTAT6750.029.2398625
ACGCAGA31000.028.6503497
ACATGGG46800.028.51693
GGTAATC3150.028.3640068
AGGTATA6750.027.8474886
CTTAGGT6800.027.6427253
TTAGGTA7150.026.2895834
CGCAGAG33650.026.2722538
TCTTAGG8600.026.2309912
Produced by FastQC (version 0.11.2)