FastQCFastQC Report
Sun 14 Apr 2019
SRR6575735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141117
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27430.24037850632319036No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23170.20304666392666135No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC18850.16518902093299812No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12540.1098923248010502No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12450.10910362390534889No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12160.10656225435253353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22050.047.8848081
TCAACGC28750.035.4754
ATCAACG29350.035.0715943
CAACGCA29250.034.8685875
AACGCAG29700.034.340286
TATCAAC33250.030.8165742
GTACATG53100.029.7377831
TACATGG54150.028.6440872
GGTATCA9300.028.3833351
GTCTTAG7850.028.2219181
ACGCAGA36350.028.0567137
ACATGGG54600.027.9775873
TAGGTAT7100.027.8029845
CGCAGAG38450.026.768828
TTAGGTA7100.026.4790334
GGTAATC3200.026.43658
CATGGGG33050.026.3087834
AGGTATA7450.025.2350546
CTTAGGT7500.025.0679173
ACCTAAG12550.024.7889731
Produced by FastQC (version 0.11.2)