FastQCFastQC Report
Sun 14 Apr 2019
SRR6575730_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575730_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031246
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14600.051.2468531
TAGTCCG555.2873816E-542.7208825
GTACATG43900.033.8723641
TACATGG44000.033.429092
TCAACGC21700.032.9167334
ATCAACG22000.032.467873
ACATGGG44800.032.3076673
CAACGCA22900.031.1918415
AACGCAG23600.030.465786
CATGGGG25700.028.5249144
TATCAAC25750.028.4695262
GTACTAG700.00878366526.8896311
GGTATCA6350.026.6779021
CTAAGAC4850.026.1610343
ACGCAGA28100.025.586927
ACCTAAG2651.8189894E-1224.8602261
CTATTAA3350.023.8795971
CGCAGAG30450.023.7827018
GGTACCT5000.022.5719498
TAAGACA6350.022.2014034
Produced by FastQC (version 0.11.2)