FastQCFastQC Report
Sun 14 Apr 2019
SRR6575730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031246
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.054.395181
TCAACGC21700.038.325674
CAACGCA22300.037.2944835
ATCAACG22550.036.8810233
AACGCAG23300.036.0971836
TATCAAC25900.032.658112
GTTAACG600.004164515531.3275972
TACATGG40650.030.1715952
GTACATG41150.029.6907961
TTACGGT953.7590835E-529.6758964
ACGCAGA28450.029.3977387
ACATGGG43800.028.4281083
GTCTATA2002.7284841E-1128.1948361
TAACGCT700.00884768626.8496194
CGCAGAG31000.026.8279678
TACGGTA950.001306625524.7299145
CTTAGGT1351.4604178E-524.3635463
CATGGGG25100.024.3358124
GCGCGAC1751.6502963E-724.1646589
AGAGTAC29500.024.0508910-11
Produced by FastQC (version 0.11.2)