FastQCFastQC Report
Sun 14 Apr 2019
SRR6575729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128811
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16100.053.8805731
TTATACG500.001701766237.6011164
TCAACGC23400.034.9497574
ATCAACG23800.034.7573363
CAACGCA24900.033.0331085
TACATGG45000.032.06542
GTACATG45250.031.7776281
AACGCAG25900.031.5762276
TATCAAC27250.030.5293462
ACATGGG47100.029.9371953
GTATAGA1303.242585E-729.0126151
GTTCTAG2550.027.7326471
ACGCAGA31950.025.4476417
ATAGTAC1151.376909E-424.5224693
CATGGGG26850.023.982094
CGCAGAG35150.023.1309288
AGAGTAC34050.022.4979410-11
CTAAGAC4750.021.7690683
TAAGACA8300.021.5187114
CCCTATA1100.00305898321.3642712
Produced by FastQC (version 0.11.2)