FastQCFastQC Report
Sun 14 Apr 2019
SRR6575727_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575727_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682307
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11140.16326961323861547No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10300.15095843952942004No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9740.14275099038995642No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9480.1389403890037769No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8830.12941388553832806No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8360.12252549072484967No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7810.11446460317716219No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7750.11358523362650537No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7250.10625715403769857No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7190.10537778448704177No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7100.10405873016105652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8250.054.2619171
ATGCGAA250.001701904837.6010570-71
TCAACGC12600.034.6939934
ATCAACG12800.034.1518973
CTAAGAC3800.033.398123
CAACGCA13650.032.022885
AACGCAG14000.031.5580276
ACCTAAG3000.031.4147931
TAAGACA3950.030.9398444
GTACATG25850.030.2602841
ATAAGGG650.006149393528.9260063
TACATGG26350.028.7201772
ACATGGG26400.027.953593
CATGGGG16500.027.6331044
CCTAAGA3650.027.0438372
ACGCAGA16900.025.8627367
GGTATCA3500.025.5806181
TATAACA950.001303272124.739352
TATCAAC18150.024.0850852
AGAGTAC17850.023.1698210-11
Produced by FastQC (version 0.11.2)