FastQCFastQC Report
Sun 14 Apr 2019
SRR6575726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362347
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9690.26742321586766277No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6550.18076595087029837No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6080.1677949589757884No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5220.14406080359434464No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5160.14240493228866252No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5150.1421289537377155No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5060.13964514677919232No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4340.11977469111100685No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4230.11673892705058964No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4050.11177131313354326No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3990.11011544182786115No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3850.10625174211460284No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3830.10569978501270881No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3660.10100814964660947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA451.6097785E-552.232961
GTATCAA9200.044.9657631
GTACATG21000.034.9214631
TTAGGTA959.432133E-734.629144
ACATGGG21400.034.259273
ACCGTGC550.002715502934.179418
AACGCAG12100.033.7910086
TACATGG21900.033.7009932
TCAACGC12050.033.5412034
CAACGCA12300.033.2415585
ATCAACG12350.032.7264373
AACCGTG600.0041586931.3311257
GAACCGT753.2725025E-431.3311256
TAGTACT902.590309E-531.3311254
ACGCAGA13650.029.9539347
AAGGGTA1303.3230208E-728.921045
TAGGTAT1154.188272E-628.6066785
CATGGGG10400.028.0172584
GTCTTAG1354.6116475E-727.8575781
CGCAGAG14700.027.8143678
Produced by FastQC (version 0.11.2)