FastQCFastQC Report
Sun 14 Apr 2019
SRR6575725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930389
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13060.14037139304097535No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12480.13413744143578654No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11650.12521644172491292No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10960.11780018895322279No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10040.10791185192430264No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG9650.10372005687943431No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9380.1008180449252947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10750.055.18871
ATCAACG16000.036.7124753
TCAACGC16300.036.036784
CAACGCA16550.035.208485
AACGCAG17100.034.0742156
GTACATG35000.031.8836781
TACATGG35000.031.2832222
ACATGGG35300.030.351753
ATACCGT6100.026.9611556
TATCAAC22400.026.8525522
TACCGTC6050.026.4101267
ACCGTCG6200.025.7891948
ACGCAGA23000.025.336167
GGTACCT5700.024.7512388
TATATGC950.001305698624.7326132
GTATAGA1151.3600301E-424.5663991
GTCTTAG2900.024.354621
CGCAGAG24650.023.8475428
GTGTAAT1401.9038078E-523.54281
CCGTCGT7250.023.3515119
Produced by FastQC (version 0.11.2)