FastQCFastQC Report
Sun 14 Apr 2019
SRR6575723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033542
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11170.10807495002622051No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10730.10381774519080986No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10630.10285019863730743No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10500.10159238811775428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13300.047.3956151
ATCAACG17350.035.4808773
TCAACGC17400.035.3789184
TAACTCG550.002719194234.1758884
CAACGCA18450.033.3654865
GTACATG38100.032.719441
ACCTAAG3950.032.1552351
TACATGG38000.032.0317542
GTCTTAG5450.031.9367141
AACGCAG20000.030.7796616
TAGGTAT3400.030.406495
AGGTATA3250.030.3639646
ACATGGG39650.030.1032283
CTTAGGT3700.027.9411013
ATAGGGT1606.926166E-826.4329153
CATGGGG24600.026.361284
TATCAAC24100.025.7407632
TAAGACA6450.025.4994534
GGTATAG3900.025.3033037
GGTATCA6300.024.6410051
Produced by FastQC (version 0.11.2)