FastQCFastQC Report
Sun 14 Apr 2019
SRR6575723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033542
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13300.12868369161582208No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12520.12113682849850321No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12500.12094331918780271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.048.6210171
TCAACGC19600.035.001974
ATCAACG19900.034.47433
CAACGCA20500.033.4652985
AACGCAG21550.032.052786
GTACATG40250.031.8784751
TACATGG41950.030.362542
ACATGGG41500.030.2314433
CATGGGG25500.027.0877464
TATCAAC25700.026.8834482
ACCTAAG4200.025.7382661
ACGCAGA27000.025.5816337
ATGGGTA6400.024.962885
CTAAGAC6450.024.0408543
CGCAGAG29050.023.9381338
GTATAAA2602.7284841E-1123.5001561
TAAGACA6650.023.3178224
TAGGTAT2853.6379788E-1223.082295
CTTAGGT2853.6379788E-1223.082293
AGAGTAC26900.022.61997610-11
Produced by FastQC (version 0.11.2)