FastQCFastQC Report
Sun 14 Apr 2019
SRR6575722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181404
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14120.1195188098228887No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13680.11579442764710463No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13560.11477868705370897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.054.820261
TCAACGC20650.042.105764
ATCAACG21000.041.4039963
CAACGCA21700.040.284975
AACGCAG23300.038.1237566
GTACATG44850.030.1864321
TACATGG45100.029.602172
TATCAAC29900.029.5575492
ACGCAGA30100.029.3549397
ACATGGG45550.029.303523
CGCAGAG32250.027.1064748
GGTATCA6000.025.8549941
GTATAGA1351.45530685E-524.3750781
GCAGAGT36400.023.8869239
AGAGTAC30200.022.95487410-11
CTATTAA2052.980414E-822.931261
CATGGGG28650.022.474284
ACCTAAG4800.021.545831
CTAAGAC8350.021.3888263
GTCTTAG6000.020.3706021
Produced by FastQC (version 0.11.2)