FastQCFastQC Report
Sun 14 Apr 2019
SRR6575708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276299
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA41610.18279672398046126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.16109482980926496No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG33020.14506002946010169No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28690.12603792384040935No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG26110.11470373619634328No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG24980.10973953773208177No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT24950.1096077448524996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.069.510561
GTATCAA65850.059.4049641
ATCAACG96050.040.5592423
TCAACGC96550.040.3978734
CAACGCA97550.039.88745
AACGCAG101750.038.3794986
TATCAAC107400.036.3612672
ACGCAGA120600.032.2638177
CGCAGAG123200.031.4920168
GTACATG141100.031.1266291
TACATGG143650.030.3915142
GCAGAGT131100.029.486719
ACATGGG151400.028.9593263
CATGGGG55700.028.6007354
ATGGGAG34300.028.223175
CATGGGA83100.026.0694944
AGAGTAC124050.025.49374410-11
CTAAGAC19650.025.3498863
ACCTAAG17750.025.1943241
AGTACTT54450.023.53459512-13
Produced by FastQC (version 0.11.2)