FastQCFastQC Report
Sun 14 Apr 2019
SRR6575708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276299
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39950.17550418464358153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.15823931741831807No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG32110.14106231211277606No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG29200.12827840279330616No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT25560.1122875334040036No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG24630.10820195413695653No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23280.10227127455575914No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23070.101348724398684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.071.70711
GTATCAA69400.058.5229151
ATCAACG96300.041.6219753
TCAACGC97950.041.016784
CAACGCA100450.039.995085
AACGCAG104000.038.7202226
TATCAAC109500.036.9195182
GTACATG146750.033.0577471
TACATGG147950.032.69432
ACGCAGA124200.032.3470657
CGCAGAG126950.031.6072648
ACATGGG156200.030.7446653
TAGGTAT7350.030.0469825
GCAGAGT133750.030.0354469
GCGCGAC7400.028.5721249
CATGGGG59850.026.9295964
CATGGGA84950.026.8826924
GGTACCT13750.026.6534618
GTCTTAG10550.026.2888281
ATGGGAG35400.026.1488935
Produced by FastQC (version 0.11.2)