FastQCFastQC Report
Sun 14 Apr 2019
SRR6575707_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575707_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499969
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43040.17216213481047166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.15776195624825748No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG36490.1459618099264431No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG29740.11896147512229151No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG27140.10856134616069239No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT26480.10592131342428647No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG25510.10204126531168987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.069.810381
GTATCAA71000.059.5529981
ATCAACG102950.041.0913433
TCAACGC104450.040.5904244
CAACGCA107400.039.6943325
AACGCAG112350.037.9036146
TATCAAC116150.036.8261532
GTACATG157800.032.29151
ACGCAGA131900.032.2104647
TACATGG159950.031.7963452
CGCAGAG135600.031.3315688
ACATGGG169550.029.8574053
GCAGAGT142700.029.6080029
CATGGGG65250.027.661534
ACCTAAG19400.026.970611
ATGGGAG40200.026.8922585
CTAAGAC20100.026.6589473
CATGGGA92000.026.5669084
AGAGTAC133650.025.93381710-11
TAAGACA26500.024.831774
Produced by FastQC (version 0.11.2)