FastQCFastQC Report
Sun 14 Apr 2019
SRR6575707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499969
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA43940.17576217945102518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.1566019418640791No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG35710.14284177123796335No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG31980.12792158622766922No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT27120.10848134516868008No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26730.10692132582444022No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA26260.10504130251215114No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA26250.105001302016145No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG25100.1004012449754377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.068.818161
GTATCAA74300.058.789091
ATCAACG107250.040.4467053
TCAACGC108200.040.048144
CAACGCA110400.039.2500845
AACGCAG113250.038.3453336
TATCAAC120000.036.1651542
GTACATG158650.032.5707321
TACATGG159800.032.277492
ACGCAGA135250.032.1080137
CGCAGAG139800.031.1302458
ACATGGG170200.030.209013
ATGGGAG41450.029.8201585
GCAGAGT147800.029.6042449
CATGGGG65950.028.2201374
CATGGGA90600.027.0264034
AGAGTAC138400.026.23258810-11
ACCTAAG18150.024.8693521
GTCTTAG10750.023.6186541
AGTACTT60800.023.61491412-13
Produced by FastQC (version 0.11.2)