FastQCFastQC Report
Sun 14 Apr 2019
SRR6575705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3342094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA351851.052783075520916No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC214760.6425911419606989No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC193890.5801452622218286No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC142050.42503292845742824No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC140590.42066440979816844No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT105880.3168073668783703No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG100700.3013081020462022No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA77540.23201023071164364No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC76560.22907793736501725No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG76530.22898817328297766No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA75560.22608580129703115No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG74610.22324327203244432No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT72050.21558340369839984No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT71240.21315977348333112No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA70670.21145425592457903No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC69150.2069062091012401No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT69140.20687628774056027No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT67200.20107154376866718No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT63960.19137702290839217No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG59580.17827146693061297No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA57520.17210766663056154No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT56730.16974387913685252No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG53980.1615155049498907No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA52910.15831391935714553No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG52890.15825407663578583No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA52480.15702730084791153No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA50160.15008554517018374No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT50030.14969656748134552No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC48590.14538789154344553No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG47660.14260520500021842No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC47220.14128866513030452No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT47060.14080992335942674No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT45800.13703983191376423No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA45660.1366209328642462No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT45650.1365910115035663No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA45410.13587289884724965No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA44860.1342272240098573No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC43790.13102563841711215No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA42740.1278838955457267No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA42670.1276744460209677No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA42310.1265972770364927No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT42110.12599884982289547No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT42090.12593900710153574No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC41620.12453270314958227No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA41610.1245027817889024No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG41290.12354529824714686No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA41190.12324608464034824No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT40620.12154056708159615No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG38950.11654369984805933No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG38820.11615472215922114No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA38010.11373109194415239No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC37230.1113972258111232No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA36400.10891375287469472No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC36000.10771689844750026No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC35820.10717831395526277No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG35040.10484444782223361No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA34320.1026901098532836No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC34210.10236097488580513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16350.062.542641
AAGGGTA18950.049.1093945
AGGGTAA21250.043.5728456
ATCAACG84450.042.744153
TCAACGC84700.042.5624964
GTGCAAG25300.042.0941931
TATCAAC86950.041.8395042
CAACGCA86500.041.513175
GTATCAA89850.041.2753641
AACGCAG89950.040.025456
GGTAATC25200.037.1132248
TGCAAGG26150.036.8465922
GTACATG144450.034.4493181
TACATGG147650.033.64782
ACGCAGA108300.032.980817
ACATGGG151750.032.645783
CAAGGGT30400.032.4683954
CGCAGAG110800.032.194248
CATGGGG107650.031.7420884
GTAATCA33650.031.424889
Produced by FastQC (version 0.11.2)